TLR4 antibody

Catalog No. ABIN119330

The Mouse Monoclonal anti-TLR4 antibody has been validated for WB, FACS, IP and Func. It is suitable to detect TLR4 in samples from Human, Dog, Pig, Guinea Pig and Monkey.

Quick Overview for TLR4 antibody (ABIN119330)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Dog, Pig, Guinea Pig, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: HTA125

Product Details anti-TLR4 Antibody

Purification: Affinity chromatography on Protein G

Endotoxin Level: < 0.01 EU/ug

Immunogen: Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: CD284 / TLR4

Background: TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4

NCBI Accession: NM_138554

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins